こんなのはどうでしょう。
<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN" "http://www.w3.org/TR/html4/loose.dtd">
<html>
<head>
<meta http-equiv="adimage" content="200">
<title>sample</title>
<style type="text/css">
<!--
iframe {
width: 300px;
height: 300px;
}
#main { float: right; }
-->
</style>
</head>
<body>
<table border="0" cellspacing="0" cellpadding="0">
<tr>
<td width="230"><img src="http://betty.jp/blanche/sample/top.gif" usemap="#top" border="0" width="230" height="500">
<map name="top">
<area shape="poly" coords="86,186,90,185,95,189,102,189,109,194,119,201,120,208,116,213,119,216,117,222,120,226,118,232,93,250,89,249,60,231,59,223,62,220,61,214,61,207,60,202,75,195,78,189" href="http://○○" target="main" alt="About">
<!-- About -->
<area shape="poly" coords="170,216,163,229,142,251,139,254,141,280,195,263,198,260,199,237,191,227,182,222" href="http://○○ target="main" alt="Priere">
<!-- Priere -->
<area shape="poly" coords="77,292,83,296,84,286,88,284,91,285,92,291,92,292,95,289,100,289,104,286,109,287,105,282,107,278,111,279,115,285,123,285,128,290,133,295,137,301,143,307,151,302,156,302,157,306,151,311,152,316,150,332,147,340,145,342,96,361,93,359,84,338,79,327,76,313,74,306,74,298" href="http://○○" target="main" alt="Footmark">
<!-- Footmark -->
<area shape="poly" coords="141,363,167,363,170,363,177,358,181,358,185,364,194,365,198,369,197,374,197,385,193,398,189,405,183,406,176,404,148,392,141,389,141,383,139,371" href="http://○○" target="main" alt="Link">
<!-- Link -->
<area shape="poly" coords="82,379,97,397,104,403,112,402,115,406,114,409,111,412,110,416,112,420,107,429,105,431,100,435,93,437,86,436,80,433,75,432,69,431,65,428,59,424,55,422,54,420,49,417,47,413,50,408,54,406,54,396,57,389,64,384,72,381" href="http://○○" target="main" alt="Top">
<!-- Top -->
<area shape="default" nohref>
<!-- デフォルトエリア -->
</map>
</td>
<td width="20">
<!--width="20"←好きな隙間の数値を指定して下さい。 -->
</td>
<td width="300" valign="bottom">
<iframe src="ファイルのURL" width="500" height="450" frameborder="0" scrolling="yes" name="main">インラインフレームを使用しています。フレームを使用しないメニューはこちらになります。</iframe>
</td>
</tr>
</table>
</body>
</html>